The Right Probiotic Strains For Young Calves
Can some of the probiotic strains reproduce and survive in 1-month-old calves? Research from Uruguay shows that it can.
The use of natural microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases affecting animals, such as neonatal calf diarrhea. Early studies have shown that probiotic-fed calves require less infectious disease treatment and the incidence and severity of neonatal calf diarrhea (NCD) can be reduced. In a study, the use of probiotics had a better preventive effect on NCD than antibiotic-based treatment. The use of probiotics is accompanied by a global trend to promote prevention and natural feeding practices. Lactic acid bacteria (LAB) are important members of the normal intestinal microbiota and are widely recognized for their beneficial effects, including the contribution to the balance of the microbiota, the inhibition of pathogen colonization, and the improvement of the host immune response.
Strains from faeces calves
The selection of probiotic strains in a collection is based on different in vitro and in vivo assays that predict their potential. The key to finding the best probiotics is that bacteria are part of the indigenous microbiota and seek the safety, durability, and adaptability of the host. Therefore, the purpose of this study published in the Journal of Probiotics is to characterize a group of natural Lactobacillus species. In vitro methods were used to isolate strains from healthy calves' feces and assess their ability to colonize calf gastrointestinal tract (GIT). Natural Lactobacillus. The strains were evaluated for their ability to survive under low pH conditions and the presence of bile salts, biofilm formation, and adhesion to mucus and Caco-2 cells
4 strains further evaluated
Based on vitro characterization, four strains (L. johnsonii TP1.1, Lactobacillus reuteri TP1.3B, Lactobacillus johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected for their assessment in calf GIT colonization and continuous ability. Assessment of intestinal persistence involves oral determination of probiotics in vivo and quantification in the feces of applied bacterial species using real-time quantitative PCR (qPCR). Fifteen 1-month-old calves were used for the study and the calves were divided into 3 groups, 4 of which were treated with 4 differently selected strains, and 1 group was the control group.
An important feature of probiotics is their resistance to the acidic environment of the stomach and the presence of bile salts in the small intestine (Morelli, 2007). In this study, it was observed that the degree of tolerance is highly variable and depends on each strain, but is not dependent on the species, which has been determined in previous studies. The results of this study indicate that in vitro screening is a powerful tool for assessing the activity of potential probiotics, particularly for determining planting capacity. Different promising strains showed significant colonization and the ability to continuously feed the calf's GIT. The researchers showed that TP1.3B and TP1.6 strains persisted in treated animals within 10 days after the end of the dosing period, indicating that they may be promising candidates for yak probiotics. Further studies will be conducted to assess their impact on the incidence of neonatal calf diarrhea (NCD) and identify potential mechanisms of action.